Plant Compounds Induce Bacterial Virulence Genes
Author Information
Author(s): Yang Shihui, Peng Quan, Michael Wang, Yongjun Wang, Zeng Quan, Yang Ching-Hong
Primary Institution: University of Wisconsin-Milwaukee
Hypothesis
Do plant phenolic compounds induce the expression of type III secretion system genes in Dickeya dadantii?
Conclusion
O-coumaric acid and t-cinnamic acid induce the expression of T3SS genes in Dickeya dadantii through the rsmB-RsmA pathway.
Supporting Evidence
- OCA and TCA significantly increased the expression of T3SS genes in Dickeya dadantii.
- The induction of T3SS expression is moderated through the rsmB-RsmA pathway.
- Promoter activity assays showed that OCA and TCA enhance hrpL mRNA levels.
Takeaway
This study found that certain plant chemicals can make bacteria more harmful by turning on their 'attack' genes.
Methodology
The study used qRT-PCR and flow cytometry to measure gene expression in bacterial cultures supplemented with plant phenolic compounds.
Limitations
The study does not explore the full range of environmental factors that may influence T3SS gene expression.
Statistical Information
P-Value
p<0.003
Statistical Significance
p<0.003
Digital Object Identifier (DOI)
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