Computational prediction of novel non-coding RNAs in Arabidopsis thaliana
2009

Predicting New Non-Coding RNAs in Arabidopsis

publication Evidence: moderate

Author Information

Author(s): Song Dandan, Yang Yang, Yu Bin, Zheng Binglian, Deng Zhidong, Lu Bao-Liang, Chen Xuemei, Jiang Tao

Primary Institution: Tsinghua University

Hypothesis

Can we effectively predict novel non-coding RNAs in the Arabidopsis genome using a comparative genomics approach?

Conclusion

The study successfully identified 21 novel non-coding RNAs in Arabidopsis that may have important biological functions.

Supporting Evidence

  • The study identified 16 families of novel ncRNAs with a total of 21 ncRNAs.
  • The identified ncRNAs are evolutionarily conserved between Arabidopsis and other recently sequenced plants.
  • The predictions were validated through wet-bench experimental methods.

Takeaway

Scientists found new types of RNA in plants that don't make proteins but help with important jobs in the cell.

Methodology

A computational pipeline was developed to predict novel ncRNAs by comparing expressed intergenic regions of Arabidopsis with other plant genomes.

Limitations

The predictions were based on computational methods and may not capture all ncRNAs present in the genome.

Digital Object Identifier (DOI)

10.1186/1471-2105-10-S1-S36

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