Single-Cell Analysis of Somatic Mutations in Human Lung Reveals Association with Transcriptional Changes in Aging
2024
Single-Cell Analysis of Lung Mutations and Aging
Sample size: 32
publication
Evidence: moderate
Author Information
Author(s): De Man Ruben, Adams Taylor, McDonough John, Cala-Garcia Juan, Moss Benjamin, Yan Xiting, Rosas Ivan, Kaminski Naftali
Primary Institution: Yale University School of Medicine
Hypothesis
Somatic mutation accumulation in lung cells is associated with transcriptional changes related to aging.
Conclusion
The study found that somatic mutation accumulation may contribute to age-related changes in lung cell function.
Supporting Evidence
- Mutation burden was highest in alveolar type 1 cells, alveolar macrophages, and general capillary cells.
- Mutation burden was positively correlated with age.
- Top genes correlated with mutation burden included ubiquitin ligase and DNA damage response genes.
- In AT1 cells, mutation burden correlated with decreased expression of cell marker genes.
- gCap cells exhibited decreased expression of certain marker genes and increased MAPK/ERK signaling genes.
Takeaway
As we get older, our lung cells collect more mutations, which can affect how they work.
Methodology
Single-cell RNA sequencing was performed on lung samples from healthy donors to analyze somatic mutations.
Participant Demographics
Healthy donors aged 11-72 years, with 21 males and 11 females.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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