High resolution crystal structure of PedB: a structural basis for the classification of pediocin-like immunity proteins
2007
Understanding the Structure of PedB, an Immunity Protein Against Pediocin PP-1
publication
Evidence: high
Author Information
Author(s): Kim In-Kwon, Kim Min-Kyu, Kim Ji-Hye, Yim Hyung-Soon, Cha Sun-Shin, Kang Sa-Ouk
Primary Institution: Seoul National University
Hypothesis
The flexible C-terminus region of PedB is critical for specific recognition of pediocin PP-1.
Conclusion
The extreme C-terminus of PedB with several charged residues is essential for immunity against pediocin PP-1.
Supporting Evidence
- The crystal structure reveals that PedB forms a compact globular domain composed of four helices.
- Deletion of the last seven residues of the C-terminus of PedB almost abolished its immunity activity.
- PedB, EntA-im, and ImB2 share a four-helix bundle structure, indicating structural conservation among pediocin-like immunity proteins.
Takeaway
PedB is a protein that helps bacteria protect themselves from their own antibiotics, and its structure is important for this protection.
Methodology
The crystal structure of PedB was determined using X-ray crystallography at a resolution of 1.6 Å.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website