Effects of JAK Inhibitor CP690,550 on T Cell Cytokine Production
Author Information
Author(s): Migita Kiyoshi, Miyashita Taiichiro, Izumi Yasumori, Koga Tomohiro, Komori Atsumasa, Maeda Yumi, Jiuchi Yuka, Aiba Yoshihiro, Yamasaki Satoshi, Kawakami Atsushi, Nakamura Minoru, Ishibashi Hiromi
Primary Institution: NHO Nagasaki Medical Center
Hypothesis
Selective inhibition of JAKs with CP690,550 would modulate T cell functions and characteristics.
Conclusion
CP690,550 inhibits the production of several cytokines from CD4+ T cells and modifies the JAK/STAT signaling pathway.
Supporting Evidence
- CP690,550 almost completely inhibited the production of IL-4, IL-17, IL-22, and IFN-γ from activated CD4+ T cells.
- CP690,550 did not affect IL-2 production from CD4+ T cells.
- CP690,550 inhibited anti-CD3-induced phosphorylation of multiple STAT proteins.
Takeaway
A medicine called CP690,550 can stop certain signals in immune cells that help them make substances called cytokines, which are important for fighting diseases.
Methodology
CD4+ T cells were stimulated with a CD3 antibody in the presence of CP690,550, and cytokine production was measured using ELISA and real-time PCR.
Participant Demographics
Healthy subjects
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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