IGF-2 Enhances Bone Formation Induced by BMP-9
Author Information
Author(s): Chen Liang, Jiang Wei, Huang Jiayi, He Bai-Cheng, Zuo Guo-Wei, Zhang Wenli, Luo Qing, Shi Qiong, Zhang Bing-Qiang, Wagner Eric R, Luo Jinyong, Tang Min, Wietholt Christian, Luo Xiaoji, Bi Yang, Su Yuxi, Liu Bo, Kim Stephanie H, He Connie J, Hu Yawen, Shen Jikun, Rastegar Farbod, Huang Enyi, Gao Yanhong, Gao Jian-Li, Zhou Jian-Zhong, Reid Russell R, Luu Hue H, Haydon Rex C, He Tong-Chuan, Deng Zhong-Liang
Primary Institution: Department of Orthopaedic Surgery, Second Affiliated Hospital, Chongqing Medical University
Hypothesis
Does insulin-like growth factor 2 (IGF-2) enhance bone formation induced by bone morphogenetic protein 9 (BMP-9)?
Conclusion
The study concludes that IGF-2 significantly enhances BMP-9-induced osteogenic differentiation and bone formation.
Supporting Evidence
- IGF-2 expression is low in mesenchymal stem cells (MSCs).
- Exogenous IGF-2 enhances BMP-9-induced alkaline phosphatase activity.
- IGF-2 augments BMP-9-induced ectopic bone formation in stem cell implantation assays.
- IGF-2 promotes endochondral ossification in limb explant cultures.
- IGFBP3 and IGFBP4 inhibit the effects of IGF-2 on BMP-9-induced osteogenic differentiation.
Takeaway
This study shows that a growth factor called IGF-2 helps another protein, BMP-9, make bones grow better, which could help heal broken bones.
Methodology
The study used various assays including stem cell implantation, limb explant culture, and biochemical assays to assess osteogenic differentiation.
Limitations
The study primarily focuses on in vitro and animal models, which may not fully replicate human conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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