A Novel Nonsense Mutation in the DMP1 Gene Identified by a Genome-Wide Association Study Is Responsible for Inherited Rickets in Corriedale Sheep
2011

Nonsense Mutation in DMP1 Causes Rickets in Corriedale Sheep

Sample size: 20 publication Evidence: high

Author Information

Author(s): Zhao Xia, Dittmer Keren E., Blair Hugh T., Thompson Keith G., Rothschild Max F., Garrick Dorian J.

Primary Institution: Iowa State University

Hypothesis

Could inherited rickets in Corriedale sheep be linked to a mutation in the DMP1 gene?

Conclusion

The R145X mutation in the DMP1 gene is responsible for inherited rickets in Corriedale sheep.

Supporting Evidence

  • The study identified a homozygous region of 125 SNP loci in all affected sheep.
  • The mutation introduced a stop codon that could truncate the DMP1 protein.
  • All affected sheep had the 'T T' genotype for the identified mutation.

Takeaway

Some sheep have a problem that makes their bones weak and deformed because of a tiny change in their genes.

Methodology

A genome-wide association study was conducted using the Illumina OvineSNP50 BeadChip on 20 related sheep, including 17 affected and 3 carriers.

Limitations

The incidence of the mutation in the Corriedale sheep population is unknown.

Participant Demographics

17 affected Corriedale sheep (9 males, 8 females) and 3 carriers.

Digital Object Identifier (DOI)

10.1371/journal.pone.0021739

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