Impact of In Vitro Culture and Cloning on SNRPN Gene in Cattle
Author Information
Author(s): Joao Suzuki, Jacinthe Therrien, France Filion, Rejean Lefebvre, Alan K Goff, Lawrence C Smith
Primary Institution: Centre de recherche en reproduction animale, University of Montreal
Hypothesis
How do in vitro culture and somatic cell nuclear transfer affect the imprinting of the SNRPN gene during cattle development?
Conclusion
Artificial reproductive techniques like in vitro culture and somatic cell nuclear transfer disrupt the normal imprinting of the SNRPN gene in cattle.
Supporting Evidence
- SNRPN gene expression was exclusively paternal in the AI group.
- IVF embryos showed bi-allelic expression at day 17.
- SCNT embryos had severe loss of DMR methylation.
- Methylation levels were significantly lower in SCNT compared to AI and IVF groups.
Takeaway
Scientists studied how different ways of making embryos affect a gene important for development in cattle. They found that some methods can mess up how this gene works.
Methodology
The study involved comparing the imprinting status of the SNRPN gene in embryos produced by artificial insemination, in vitro fertilization, and somatic cell nuclear transfer.
Potential Biases
Potential bias in the selection of embryos and the methods used for in vitro culture and cloning.
Limitations
The study primarily focused on a specific gene and may not represent broader implications for all imprinted genes in cattle.
Participant Demographics
Cattle embryos from Bos indicus and Bos taurus breeds were used.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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