How Gonadotropins Regulate Estrogen Production in Rat Ovaries
Author Information
Author(s): Yoshihiko Inaoka, Takashi Yazawa, Tetsuya Mizutani, Koichi Kokame, Kenji Kangawa, Miki Uesaka, Akihiro Umezawa, Kaoru Miyamoto
Primary Institution: University of Fukui
Hypothesis
The regulation of P450 oxidoreductase (POR) expression in ovarian granulosa cells by gonadotropins is important for steroidogenesis.
Conclusion
Gonadotropins regulate the expression of POR and aromatase, which together enhance estrogen production in the ovary.
Supporting Evidence
- POR mRNA expression was significantly increased by gonadotropin treatment in rat ovaries.
- Transient expression of wild-type POR proteins greatly enhanced the conversion of androstenedione to estrone.
- Knockdown of POR in KGN cells reduced estrone production, indicating its role in estrogen synthesis.
Takeaway
This study shows that certain hormones help the ovaries make more estrogen by increasing the levels of important proteins.
Methodology
The study used DNA microarray analysis, RT-PCR, and western blotting to examine gene expression changes in rat ovarian cells.
Participant Demographics
Rat ovarian granulosa cells were used in the study.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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