GAPDH Expression in Tumor Cells Under Hypoxia
Author Information
Author(s): Said Harun M, Polat Buelent, Hagemann Carsten, Anacker Jelena, Flentje Michael, Vordermark Dirk
Primary Institution: University of Würzburg
Hypothesis
Is GAPDH expression regulated by hypoxia in tumor cells of different origins?
Conclusion
GAPDH is a suitable housekeeping gene for studying hypoxia-induced gene expression in various tumors, as it does not show regulation under hypoxic conditions.
Supporting Evidence
- GAPDH expression was not regulated by hypoxia in various cancer cell lines.
- The study confirmed previous findings that GAPDH is not an attractive target for tumor therapies under hypoxic conditions.
- GAPDH can serve as a reliable loading control in experiments involving glioma and other cancer cells.
Takeaway
GAPDH is like a reliable friend that doesn't change no matter how tough things get, making it a good choice for scientists studying cancer.
Methodology
The study used in vitro cell culture assays with various cancer cell lines to assess GAPDH expression under hypoxic conditions using RT-PCR and western blot techniques.
Limitations
The study did not measure oxygenation levels directly in human tumors.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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