How SRC3 and 53BP1 Regulate the BRCA1 Gene
Author Information
Author(s): Corkery Dale, Thillainadesan Gobi, Coughlan Niamh, Mohan Ryan D, Isovic Majdina, Tini Marc, Torchia Joseph
Primary Institution: Department of Oncology, London Regional Cancer Program and the Lawson Health Research Institute
Hypothesis
The SRC3/53BP1 complex regulates the expression of the BRCA1 gene.
Conclusion
SRC3 and 53BP1 are both required for the expression of the BRCA1 gene, suggesting their complex plays a role in DNA repair.
Supporting Evidence
- SRC3 and 53BP1 co-occupy the same region of the BRCA1 promoter.
- Knockdown of either SRC3 or 53BP1 significantly decreases BRCA1 protein levels.
- SRC3 is not directly involved in the DNA damage response.
Takeaway
SRC3 and 53BP1 work together to help turn on a gene called BRCA1, which is important for fixing DNA.
Methodology
The study used protein purification, mass spectrometry, chromatin immunoprecipitation (ChIP), and siRNA knockdown techniques.
Limitations
The study did not explore the role of SRC3 in other DNA damage response mechanisms.
Digital Object Identifier (DOI)
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