First-Pass Myocardial Perfusion MRI in Mice
Author Information
Author(s): Marcus Makowski, Christian Jansen, Ian Webb, Amedeo Chiribiri, Eike Nagel, Rene Botnar, Sebastian Kozerke, Sven Plein
Primary Institution: King's College London
Hypothesis
Can first-pass contrast-enhanced myocardial perfusion MRI be successfully performed in mice using a clinical 3-T MR scanner?
Conclusion
The study successfully demonstrates a novel method for first-pass myocardial perfusion MRI in mice, providing accurate myocardial blood flow measurements.
Supporting Evidence
- First-pass myocardial perfusion imaging was successfully performed in all nine mice.
- Control mice showed a mean myocardial blood flow of 7.3 ± 1.5 mL/g/min.
- Infarcted segments had significantly reduced myocardial blood flow of 1.2 ± 0.8 mL/g/min.
- Signal-intensity-time profiles indicated a percentage myocardial signal increase of 141.3 ± 38.9% in normal mice.
Takeaway
Researchers figured out how to take pictures of blood flow in mouse hearts using a special MRI technique, which helps us understand heart problems better.
Methodology
The study used a custom pulse sequence on a clinical 3.0-T MR scanner to perform first-pass myocardial perfusion imaging in healthy and infarcted mice.
Limitations
The contrast delivery was manual and could be automated; only one slice was acquired at each RR interval, and the optimal contrast dose needs further optimization.
Participant Demographics
Nine 10- to 12-week-old homozygous C57BL/6J male mice were used, with five as healthy controls and four with induced myocardial infarction.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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