Adenovirus Type 5 and Its Effects on Cell Growth
Author Information
Author(s): Daniel L. Miller, Chad L. Myers, Brenden Rickards, Hilary A. Coller, S. Jane Flint
Primary Institution: Princeton University
Hypothesis
What are the effects of adenovirus type 5 (Ad5) infection on cellular gene expression in normal human fibroblasts?
Conclusion
Adenovirus infection significantly alters host cell gene expression, reversing quiescence and promoting proliferation.
Supporting Evidence
- About 2,000 genes were up- or down-regulated after Ad5 infection.
- Ad5 infection induces reversal of the quiescence program.
- Gene Ontology terms associated with cell proliferation were significantly over-represented.
Takeaway
When a virus called adenovirus type 5 infects human cells, it changes how many genes work, making the cells grow and divide instead of resting.
Methodology
Microarray analysis of human fibroblasts was conducted to monitor changes in gene expression after Ad5 infection.
Potential Biases
Potential bias in gene expression analysis due to the specific conditions of the cell culture.
Limitations
The study primarily focuses on a single type of human cell and may not generalize to other cell types.
Participant Demographics
Normal human fibroblasts were used in the study.
Statistical Information
P-Value
< 10-10
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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