Calcium Entry in Activated Human Neutrophils
Author Information
Author(s): Pan Leiting, Wu Xian Zhao, Dan Hessari, Nason Ma’ani, Lee Imshik Zhang, Xinzheng Xu, Jingjun
Primary Institution: The MOE Key Laboratory of Weak-Light Nonlinear Photonics, TEDA Applied Physics School and School of Physics, Nankai University, Tianjin, People’s Republic of China
Hypothesis
Does sulfhydryl modification induce calcium entry through an IP3-sensitive store-operated pathway in activated human neutrophils?
Conclusion
Sulfhydryl modification by NEM and SNP induces calcium entry in PMA-activated human neutrophils through an IP3-sensitive store-operated pathway.
Supporting Evidence
- NEM and SNP induced a rapid increase in calcium concentration in PMA-activated neutrophils.
- Calcium entry was dependent on the depletion of calcium stores.
- 2-APB effectively blocked the calcium entry induced by NEM and SNP.
Takeaway
When certain chemicals modify proteins in activated immune cells called neutrophils, it helps them take in calcium, which is important for their function.
Methodology
The study involved measuring calcium levels in human neutrophils after treatment with NEM and SNP, using fluorescence microscopy and spectrofluorometry.
Limitations
The study primarily focused on PMA-activated neutrophils and may not generalize to other activation states or cell types.
Participant Demographics
Human neutrophils obtained from healthy adults.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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