Understanding How Herpes Simplex Virus Helps Adeno-Associated Virus Replicate
Author Information
Author(s): Alazard-Dany Nathalie, Nicolas Armel, Ploquin Aurélie, Strasser Regina, Greco Anna, Epstein Alberto L., Fraefel Cornel, Salvetti Anna
Primary Institution: INSERM U758, Lyon, France
Hypothesis
What are the specific helper activities of Herpes Simplex Virus Type 1 that facilitate the early replication events of Adeno-Associated Virus?
Conclusion
The study identifies nine HSV-1 proteins that are essential for the early steps of AAV replication, including the activation of the rep gene and AAV DNA replication.
Supporting Evidence
- The study confirmed the role of ICP0 for rep gene expression.
- A synergistic effect of ICP4 and ICP22 on rep gene expression was demonstrated.
- The HSV-1 DNA polymerase complex was shown to enhance AAV DNA replication significantly.
Takeaway
This study shows that a virus called Herpes Simplex Virus helps another virus, Adeno-Associated Virus, to make copies of itself by using specific proteins.
Methodology
The study used a cellular model mimicking AAV latency and composite constructs coding for various HSV-1 genes to analyze their roles in AAV replication.
Digital Object Identifier (DOI)
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