Application of Pulsed-Field Gel Electrophoresis to an International Outbreak of Salmonella agona
1996

Salmonella agona Outbreak Linked to Contaminated Snacks

Sample size: 78 publication Evidence: high

Author Information

Author(s): E. John Threlfall, Michael D. Hampton, Linda R. Ward, Bernard Rowe

Primary Institution: Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, United Kingdom

Hypothesis

Can pulsed-field gel electrophoresis (PFGE) effectively identify and trace the source of Salmonella agona infections during an outbreak?

Conclusion

The study identified a specific strain of Salmonella agona responsible for outbreaks in multiple countries, linked to a contaminated snack.

Supporting Evidence

  • Isolates from the contaminated snack and patients who consumed it all belonged to the same Salmonella agona phage type.
  • PFGE analysis revealed 11 distinct profiles among the isolates.
  • The predominant PFGE profile was found in 51 of the 78 isolates examined.

Takeaway

Scientists found that a type of Salmonella that made people sick came from a snack that was shared across different countries.

Methodology

The study used pulsed-field gel electrophoresis (PFGE) to analyze 78 isolates of Salmonella agona from various countries.

Limitations

PFGE typing may not be applicable to all salmonella serotypes and phage types.

Participant Demographics

Most isolates were from children under 10 years of age, particularly Jewish children in London.

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