Cigarette Smoke and Neutrophil Migration
Author Information
Author(s): Saskia A. Overbeek, Saskia Braber, Paul A. J. Henricks, Marije Kleinjan, Vera M. Kamp, Niki A. Georgiou, Johan Garssen, Aletta D. Kraneveld, Gert Folkerts
Primary Institution: Utrecht University, Utrecht, The Netherlands
Hypothesis
Does cigarette smoke induce neutrophil migration and activate β2-integrins in neutrophilic transmigration through endothelium?
Conclusion
Cigarette smoke extract induces a direct migratory effect on neutrophils and activates β2-integrins, which may be a target for treating neutrophilic diseases.
Supporting Evidence
- Cigarette smoke extract (CSE) dose dependently induced migration of neutrophils in vitro.
- CSE promoted neutrophil adherence to fibrinogen.
- Blocking antibodies against CD11b and CD18 inhibited neutrophil adhesion and transmigration.
- Neutrophils transmigrated through endothelium due to activation of β2-integrins by CSE.
Takeaway
Cigarette smoke makes certain white blood cells, called neutrophils, move more easily through blood vessels, which can lead to lung problems.
Methodology
The study used freshly isolated human neutrophils to assess the effects of cigarette smoke extract on migration and β2-integrin activation.
Participant Demographics
38.1% men and 61.9% women, median age 48 (range: 27-60)
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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