Mutation of L-2,3-diaminopropionic acid synthase genes blocks staphyloferrin B synthesis in Staphylococcus aureus
2011
Blocking Staphyloferrin B Synthesis in Staphylococcus aureus
publication
Evidence: high
Author Information
Author(s): Beasley Federico C, Cheung Johnson, Heinrichs David E
Primary Institution: University of Western Ontario
Hypothesis
The sbnA and sbnB genes are essential for the synthesis of staphyloferrin B in Staphylococcus aureus.
Conclusion
Mutations in sbnA or sbnB prevent the synthesis of staphyloferrin B, which is crucial for the iron acquisition of S. aureus.
Supporting Evidence
- Mutants lacking sbnA or sbnB showed no growth under iron-limiting conditions without supplementation.
- Supplementation with L-2,3-diaminopropionic acid restored staphyloferrin B production in mutants.
- Electrospray ionization-mass spectrometry confirmed the absence of staphyloferrin B in mutant strains.
Takeaway
The study found that two specific genes in Staphylococcus aureus are needed to make a special molecule that helps the bacteria get iron, which is important for their growth.
Methodology
Genetic manipulation and growth assays were performed to assess the role of sbnA and sbnB in staphyloferrin B synthesis.
Digital Object Identifier (DOI)
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