Establishment of Human Papillomavirus Infection Requires Cell Cycle Progression Host Cell Mitosis Is Critical for HPV Infection
2009

How Cell Division Affects HPV Infection

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Author Information

Author(s): Pyeon Dohun, Pearce Shane M., Lank Simon M., Ahlquist Paul, Lambert Paul F.

Primary Institution: University of Wisconsin-Madison

Hypothesis

Cell cycle progression through mitosis is critical for HPV infection.

Conclusion

The study concludes that HPV can only establish infection in host cells that are undergoing cell division, specifically during early prophase of mitosis.

Supporting Evidence

  • HPV infection requires host cells to be in the M phase of the cell cycle.
  • Cell cycle inhibitors were effective in blocking HPV infection.
  • Only undifferentiated proliferating cells can be infected by HPV.

Takeaway

HPV needs the host cells to be in a specific stage of division to infect them, like needing a door to be open to get inside a house.

Methodology

The study used chemical biology approaches to test the effects of various cell cycle inhibitors on HPV infection in human keratinocyte cells.

Limitations

The study primarily used immortalized cell lines, which may not fully represent primary keratinocytes in vivo.

Participant Demographics

The study involved human keratinocyte cell lines, specifically 293T and HaCaT cells.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.ppat.1000318

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