Identification of two proteins that interact with the Erp virulence factor from Mycobacterium tuberculosis by using the bacterial two-hybrid system
2009

Identifying Proteins that Interact with Mycobacterium tuberculosis Erp

publication Evidence: moderate

Author Information

Author(s): Klepp Laura I, Soria Marcelo, Blanco Federico C, Bianco María V, Santangelo María P, Cataldi Angel A, Bigi Fabiana

Primary Institution: Institute of Biotechnology, CICVyA-INTA Castelar

Hypothesis

The central repeat regions of the Erp protein participate in interactions with other proteins.

Conclusion

The study identified two proteins, Rv1417 and Rv2617c, that interact with the Erp protein, which is involved in the virulence of Mycobacterium tuberculosis.

Supporting Evidence

  • Erp is a virulence factor for Mycobacterium tuberculosis.
  • Rv1417 and Rv2617c were identified as interacting partners of Erp.
  • The interactions were confirmed using a bacterial two-hybrid system.

Takeaway

Researchers found two proteins that work with a key protein in tuberculosis bacteria, which helps the bacteria survive and cause disease.

Methodology

The study used a bacterial two-hybrid system to identify protein interactions.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2199-10-3

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