Requirement of focal adhesion kinase in branching tubulogenesis
2009
Focal Adhesion Kinase and Tubulogenesis
publication
Evidence: moderate
Author Information
Author(s): Wei Wei-Chun, Kopec Anna K, Tang Ming-Jer
Primary Institution: National Cheng Kung University Medical College
Hypothesis
FAK is required for HGF-independent branching tubulogenesis in MDCK cells.
Conclusion
FAK is necessary but not sufficient for HGF-independent branching tubulogenesis in MDCK cells.
Supporting Evidence
- Mass clones developed into tubular structures while cyst clones maintained cystic morphology.
- FAK expression was higher in mass clones than in cyst clones.
- Decreasing FAK expression in mass clones reduced tubule formation.
Takeaway
FAK helps cells form tube-like structures, but just having FAK isn't enough to make it happen.
Methodology
The study involved isolating MDCK cell clones and analyzing their morphogenesis in collagen gel.
Limitations
The study primarily focused on in vitro models, which may not fully represent in vivo conditions.
Digital Object Identifier (DOI)
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