Using Laparoscopy to Study Liver Disease in Mice
Author Information
Author(s): Yami Shapira, Meirav Katz, Muhammad Ali, Michael Kaplan, Eli Halpern, Zamir Elinav, Eran Elinav
Primary Institution: Institute for Gastroenterology and Liver Disease, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel
Hypothesis
Can a miniaturized endoscopy system be used for safe and repeated liver biopsies in mice?
Conclusion
Murine laparoscopy is a novel imaging technique that allows for continuous assessment and manipulation of chronic liver disease models.
Supporting Evidence
- The laparoscopic technique allows for repeated visualization of liver disease progression.
- Biopsies taken during laparoscopy provide adequate tissue for histological analysis.
- Tumor cells can be accurately implanted into the liver using this method.
Takeaway
Researchers found a way to look at mouse livers without having to kill the mice, which helps them see how diseases change over time.
Methodology
The study involved using a miniaturized endoscopy system to perform laparoscopic liver biopsies and tumor cell injections in mice.
Limitations
There is a low mortality rate associated with the procedure, particularly during the learning curve phase.
Participant Demographics
8–10 week-old female C57BL/6 and Balb C mice and Wister rats.
Digital Object Identifier (DOI)
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