Mucin 1 (MUC1) is a novel partner for MAL2 in breast carcinoma cells
2009
MUC1 as a Partner for MAL2 in Breast Cancer Cells
Sample size: 5000000
publication
Evidence: moderate
Author Information
Author(s): Fanayan Susan, Shehata Mona, Agterof Annelies P, McGuckin Michael A, Alonso Miguel A, Byrne Jennifer A
Primary Institution: The Children's Hospital at Westmead
Hypothesis
Does MAL2 interact with MUC1 in breast carcinoma cells?
Conclusion
MUC1 is identified as a novel partner for MAL2, suggesting that MAL2 may regulate MUC1 expression and localization.
Supporting Evidence
- MAL2 was found to be overexpressed in breast cancer.
- Yeast two-hybrid screening identified MUC1 as a frequent binding partner of MAL2.
- Co-immunoprecipitation confirmed the interaction between MAL2 and MUC1 in breast cancer cells.
- Confocal microscopy showed co-localization of MAL2 and MUC1 in the cytoplasm of breast cancer cells.
Takeaway
The study found that a protein called MUC1 works together with another protein called MAL2 in breast cancer cells, which might help MUC1 get to the cell surface.
Methodology
Yeast two-hybrid screening and co-immunoprecipitation analyses were used to identify and confirm interactions between MAL2 and MUC1.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website