Validation of Serum Biomarkers for Breast Cancer
Author Information
Author(s): Annemieke WJ van Winden, Marie-Christine Gast, Jos H Beijnen, Emiel JTh Rutgers, Diederick E Grobbee, Petra HM Peeters, Carla H van Gils
Primary Institution: University Medical Center Utrecht
Hypothesis
This study aims to validate three proteins previously reported to be discriminative between breast cancer cases and healthy controls.
Conclusion
The study found that two proteins were decreased in breast cancer patients, aligning with most previous studies, while one protein showed no relation to breast cancer.
Supporting Evidence
- Two proteins were found to be statistically significantly decreased in breast cancer cases compared to healthy controls.
- The intensity of the peak with an m/z of 8129 showed no difference between cases and controls.
- Adjustment for sample preparation day and storage duration did not substantially alter results.
Takeaway
The researchers looked at blood samples from women with breast cancer and healthy women to see if certain proteins could help detect the disease.
Methodology
Serum protein profiles of 48 breast cancer patients and 48 healthy controls were analyzed using SELDI-TOF MS, with statistical analysis performed using the Mann-Whitney U test and multivariate logistic regression.
Potential Biases
Potential biases due to differences in sample handling and storage conditions.
Limitations
Differences in patient populations and pre-analytical sample handling could have contributed to discrepancies in results.
Participant Demographics
Participants included 48 women diagnosed with primary invasive breast cancer and 48 healthy controls, matched for age.
Statistical Information
P-Value
<0.001
Confidence Interval
0.61–0.82
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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