Normalizing Factors for Gene Expression in Brain Injury Studies
Author Information
Author(s): Hervé Rhinn, Catherine Marchand-Leroux, Nicole Croci, Michel Plotkine, Daniel Scherman, Virginie Escriou
Primary Institution: Inserm, U640, Paris, France
Hypothesis
What are the optimal normalizing factors for RT-qPCR in a murine model of traumatic brain injury?
Conclusion
Oligreen cDNA measurements, 18S rRNA, and GAPDH are suggested as effective normalizing factors for gene expression studies in mouse brain trauma.
Supporting Evidence
- Oligreen cDNA measurements ranked among the best normalizing factors.
- GAPDH and 18S rRNA were also found to be suitable for normalization.
- β-actin and β-microtubulin should be avoided as normalizing factors.
Takeaway
This study found that certain measurements can help scientists better understand gene expression after brain injuries in mice.
Methodology
The study compared five potential reference genes and total cDNA levels using various statistical methods to determine the best normalizing factors for qRT-PCR.
Potential Biases
Potential bias in selecting reference genes without prior validation.
Limitations
The study focused only on a specific murine model and may not generalize to other models or conditions.
Participant Demographics
Male CD-1 mice, weighing 21–24 g.
Statistical Information
P-Value
<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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