Automated Colorimetric Analysis of Fructose
Author Information
Author(s): R. B. Roy, A. Buccafuri
Primary Institution: Technicon Industrial Systems
Hypothesis
An improved automated procedure for analyzing fructose in fermentation media can retain accuracy while using milder reaction conditions.
Conclusion
The new automated method for analyzing fructose is rapid, accurate, and can process twenty samples per hour.
Supporting Evidence
- The method can analyze fructose in the range of 0.01-0.1 g/l solution.
- The total elapsed time from sample introduction to readout is 6 minutes.
- Recovery of fructose in real samples ranged from 95% to 101%.
Takeaway
This study shows a new way to quickly check how much fructose is in a liquid, which is important for making sweet syrups.
Methodology
The method involves using cysteine, carbazole, and sulphuric acid in a continuous-flow analytical system to measure fructose concentration.
Limitations
The method may have variations in sensitivity compared to manual procedures and requires careful calibration.
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