In Mycoplasma hominis the OppA-mediated cytoadhesion depends on its ATPase activity
2011
How Mycoplasma hominis Sticks to Cells
publication
Evidence: moderate
Author Information
Author(s): Miriam Hopfe, Theresa Dahlmanns, Birgit Henrich
Primary Institution: Heinrich-Heine-University Duesseldorf
Hypothesis
Does the ATPase activity of OppA influence the cytoadhesion of Mycoplasma hominis?
Conclusion
The study concludes that the cytoadhesion of Mycoplasma hominis is dependent on the ATPase activity of the OppA protein.
Supporting Evidence
- Mutations in the Walker BA motifs reduced ATPase activity significantly.
- Deletion of specific regions in the OppA protein affected its ability to adhere to HeLa cells.
- ATPase inhibitors reduced the cytoadhesion of Mycoplasma hominis to HeLa cells.
Takeaway
Mycoplasma hominis uses a special protein called OppA to stick to cells, and this sticking depends on the protein's ability to break down ATP for energy.
Methodology
The study involved creating mutants of the OppA protein and testing their ATPase activity and ability to adhere to HeLa cells.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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