Molecular Biomarkers for Bacterial Cell Viability
Author Information
Author(s): Kort Remco, Keijser Bart J, Caspers Martien PM, Schuren Frank H, Montijn Roy
Primary Institution: TNO Quality of Life, Business Unit Food and Biotechnology Innovations, Microbial Genomics Group
Hypothesis
The study investigates the correlation between transcriptional activity, membrane integrity, and cultivation-based viability in Bacillus subtilis.
Conclusion
Transcription of a limited number of genes correlated with cell viability under heat stress, and these genes can serve as molecular biomarkers for bacterial cell viability.
Supporting Evidence
- The study identified a coherent transcriptional program in Bacillus subtilis under heat stress.
- Specific gene transcripts were found to correlate with cell viability.
- Transcriptional activity continued even after the bacteria lost the ability to reproduce.
Takeaway
The study shows that even after bacteria die from heat, some genes are still active, which can help scientists figure out if bacteria are alive or dead.
Methodology
The study used microbiological, cytological, and molecular analyses to assess the effects of lethal heat stress on Bacillus subtilis.
Limitations
The study primarily focuses on a single bacterial species and specific heat stress conditions, which may limit the generalizability of the findings.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website