Human Mena Associates with Rac1 in Glioblastoma Cells
Author Information
Author(s): Higashi Morihiro, Ishikawa Chieko, Yu Jianyong, Toyoda Akihiro, Kawana Hidetada, Kurokawa Kazuo, Matsuda Michiyuki, Kitagawa Motoo, Harigaya Kenichi
Primary Institution: Chiba University Graduate School of Medicine
Hypothesis
hMena is a component of the Rac interacting proteins.
Conclusion
Depleting hMena leads to increased Rac1 activity and the formation of lamellipodia in glioblastoma cells.
Supporting Evidence
- hMena colocalizes with Rac1 in lamellipodia.
- Depletion of hMena leads to increased Rac1 activation.
- Introduction of dominant negative Rac1 cancels the effects of hMena depletion.
- Similar effects were observed in U373MG cells but not in HeLaS3 cells.
Takeaway
When a protein called hMena is reduced in certain brain cancer cells, those cells spread out more and form structures called lamellipodia, which help them move.
Methodology
The study used siRNA to deplete hMena in glioblastoma cell lines and analyzed the effects on cell morphology and Rac1 activity.
Limitations
The study's findings may not apply to all cell types, as the effects were not observed in HeLaS3 cells.
Participant Demographics
The study focused on glioblastoma cell lines U251MG and U373MG.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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