Genomic Analysis of Secretion Stress in Aspergillus niger
Author Information
Author(s): Guillemette Thomas, van Peij Noël NME, Goosen Theo, Lanthaler Karin, Robson Geoffrey D, van den Hondel Cees AMJJ, Stam Hein, Archer David B
Primary Institution: School of Biology, University of Nottingham
Hypothesis
This study aims at uncovering transcriptional and translational responses occurring in A. niger exposed to secretion stress.
Conclusion
The study provides insight into the molecular basis of protein secretion and secretion-related stress in Aspergillus niger, identifying target genes for strain improvement strategies.
Supporting Evidence
- The study is the first genome-wide analysis of both transcriptional and translational events following protein secretion stress.
- Insight has been gained into the molecular basis of protein secretion and secretion-related stress in an effective protein-secreting fungus.
- Several genes were down-regulated under stress conditions, including those encoding secreted enzymes.
Takeaway
Scientists studied how a fungus called Aspergillus niger reacts when it has trouble sending out proteins. They found out which genes help it deal with this stress.
Methodology
A genome-wide transcriptional analysis was performed using Affymetrix DNA GeneChips, with verification for selected genes through Northern hybridization and RT-PCR.
Limitations
The study did not include strains of A. niger that are devoid of key mediators of the UPR, limiting the analysis of certain stress responses.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website