Mononuclear Cell Therapy Reduces Atherosclerosis in Mice
Author Information
Author(s): Marcella L Porto, Leandro CF Lima, Thiago MC Pereira, Breno V Nogueira, Clarissa L Tonini, Bianca P Campagnaro, Silvana S Meyrelles, Elisardo C Vasquez
Primary Institution: Federal University of Espirito Santo
Hypothesis
MNC therapy attenuates the progression of vascular atherosclerosis through the homing of EPCs, reducing reactive oxygen species (ROS), and increasing the expression of endothelial nitric oxide synthase (eNOS).
Conclusion
MNC therapy attenuates the progression of atherosclerosis in the aortas of apoE KO mice.
Supporting Evidence
- Histological analysis showed a significant reduction in lipid deposition in treated mice.
- MNC therapy prevented outward remodeling in the aortas of apoE KO mice.
- Treated mice had reduced superoxide production and increased eNOS expression.
- Endothelial progenitor cells were found to home in the aortas of treated mice.
Takeaway
This study shows that giving special cells from the spleen to mice with heart problems helps them get better by reducing bad stuff in their blood vessels.
Methodology
ApoE KO mice were treated with mononuclear cells from lacZ transgenic mice for 8 weeks, and various vascular parameters were measured.
Potential Biases
Potential bias in the interpretation of histological results due to the blinding of the examiner.
Limitations
The study was conducted in a mouse model, which may not fully replicate human atherosclerosis.
Participant Demographics
ApoE KO female mice, 24 weeks old.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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