Nutlin-3a Enhances Reovirus-Induced Tumor Cell Death
Author Information
Author(s): Pan D, Pan L-Z, Hill R, Marcato P, Shmulevitz M, Vassilev L T, Lee P W K
Primary Institution: Dalhousie University
Hypothesis
Does stabilisation of p53 enhance reovirus-induced apoptosis and virus spread through p53-dependent NF-κB activation?
Conclusion
Nutlin-3a treatment enhances reovirus-induced apoptosis and virus spread through p53-dependent NF-κB activation.
Supporting Evidence
- Nutlin-3a significantly increased reovirus-induced apoptosis in p53+/+ cells.
- Reovirus replication was similar in p53+/+ and p53−/− cells, but apoptosis was enhanced in p53+/+ cells with Nutlin-3a.
- Nuclear translocation of NF-κB was enhanced by Nutlin-3a in p53+/+ cells.
- Combination treatment resulted in larger reovirus plaques, indicating enhanced virus spread.
Takeaway
This study shows that a drug called Nutlin-3a can help a virus that kills cancer cells work better by making the cancer cells die more and spread the virus.
Methodology
Human cancer cell lines were treated with Nutlin-3a, reovirus, or both, followed by various assays to measure reovirus titration, apoptosis, and NF-κB activation.
Potential Biases
Potential bias in the selection of cell lines and treatments used.
Limitations
The study primarily focuses on specific cancer cell lines and may not generalize to all cancer types.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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