Barrier-to-Autointegration Factor (BAF) Does Not Bind to HIV-1 MA, Crx, or MAN1-C Without DNA
Author Information
Author(s): Huang Ying, Cai Mengli, Clore G. Marius, Craigie Robert
Primary Institution: National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health
Hypothesis
Does BAF interact directly with HIV-1 MA, Crx, or MAN1-C?
Conclusion
BAF does not directly interact with HIV-1 MA, Crx, or MAN1-C, and previous reports of such interactions are likely due to indirect associations through DNA binding.
Supporting Evidence
- BAF does not interact directly with HIV-1 MA, Crx, or MAN1-C.
- The interactions reported in previous studies are likely mediated through DNA binding.
- NMR spectroscopy showed no significant changes in spectra indicating no direct interactions.
Takeaway
BAF is a protein that helps protect viral DNA, but it doesn't actually stick to some other proteins like HIV-1 MA, Crx, or MAN1-C unless DNA is around.
Methodology
The study used NMR spectroscopy to monitor interactions between BAF and its putative binding partners.
Limitations
The study primarily focused on specific protein interactions and did not explore other potential interactions or biological contexts.
Digital Object Identifier (DOI)
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