The Role of Zebrafish Ext2 in Fgf, Wnt, and Hh Signaling
Author Information
Author(s): Sabine Fischer, Beata Filipek-Gorniok, Johan Ledin
Primary Institution: European Molecular Biology Laboratory (EMBL)
Hypothesis
Ext2 is necessary for Fgf and Wnt signaling but not for Hh signaling during zebrafish development.
Conclusion
Ext2 is involved in Fgf and Wnt signaling but not in Hh signaling, indicating specific roles for heparan sulfate biosynthesis in different signaling pathways.
Supporting Evidence
- Fgf signaling is reduced in ext2 mutants, affecting gene expression.
- Wnt signaling is also impacted in ext2 mutants, showing stronger phenotypes with reduced Wnt translation.
- Hh signaling remains unaffected in ext2 mutants, indicating a specific role for ext2.
Takeaway
This study shows that a gene called ext2 helps certain signals in zebrafish grow and develop, but it doesn't help with another type of signal.
Methodology
The study involved examining ext2 mutants and their response to various signaling pathways during zebrafish development.
Limitations
The study primarily focuses on zebrafish and may not directly translate to other species.
Participant Demographics
Zebrafish embryos were used in the study.
Digital Object Identifier (DOI)
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