Study of Paracrine Factors in a Tumor-Bone Coculture Model
Author Information
Author(s): Katherine R Schiller, Marion R Zillhardt, Jeremy Alley, Dori L Borjesson, Alvin J Beitz, Laura J Mauro
Primary Institution: University of Minnesota
Hypothesis
The study aims to test an ex vivo coculture model that will allow monitoring of the expression, release, and regulation of paracrine factors during interactions of an intact femur explant and tumor cells.
Conclusion
The study demonstrates the feasibility of using a coculture model to examine paracrine interactions between intact bone and tumor cells, revealing unique regulation of MCP-1 secretion.
Supporting Evidence
- The coculture model allows for the examination of paracrine interactions between bone and tumor cells.
- MCP-1 secretion was significantly enhanced in cocultures compared to single component cultures.
- Marrow depletion reduced MCP-1 secretion from both bone and cocultures.
Takeaway
Researchers created a model to study how tumor cells and bone interact, finding that they can influence each other's behavior, especially in the release of a chemical called MCP-1.
Methodology
The study used intact or marrow-depleted neonatal mouse femurs and various murine and human sarcoma or carcinoma cell lines in coculture to assess viability and secretion of paracrine factors.
Limitations
The model primarily investigates early paracrine interactions and does not account for chronic exposure effects; the neonatal femurs used may not fully represent adult bone.
Participant Demographics
Neonatal CD-1 strain mice were used for femur dissection.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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