Identifying RBP-J as a DNA Methylation Binding Protein
Author Information
Author(s): Bartels Stefanie J. J., Spruijt Cornelia G., Brinkman Arie B., Jansen Pascal W. T. C., Vermeulen Michiel, Stunnenberg Hendrik G.
Primary Institution: Radboud University Nijmegen, Nijmegen, The Netherlands
Hypothesis
Can a SILAC-based approach identify novel methyl-CpG binding proteins?
Conclusion
The study successfully identifies RBP-J as a methylation-dependent binder to DNA, highlighting its role in the methyl-DNA interactome.
Supporting Evidence
- The study identified several known methyl-CpG binding proteins using a novel SILAC-based approach.
- RBP-J was found to bind methylated DNA in a sequence-specific manner.
- Follow-up experiments confirmed the binding of RBP-J to methylated DNA.
Takeaway
Scientists found a new way to see how certain proteins stick to DNA that has special chemical tags, and they discovered a protein called RBP-J that does this in a specific way.
Methodology
The study used a methyl-CpG pull-down assay combined with stable-isotope labeling by amino acids in cell culture (SILAC) to identify methyl-CpG binding proteins.
Limitations
The study may not have identified all potential methyl-CpG binding proteins due to the specific conditions and sequences used.
Digital Object Identifier (DOI)
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