Heterologous prime-boost-boost immunisation of Chinese cynomolgus macaques using DNA and recombinant poxvirus vectors expressing HIV-1 virus-like particles
2011

HIV Vaccine Study in Macaques

Sample size: 3 publication Evidence: low

Author Information

Author(s): Simon Bridge, Sally A Sharpe, Mike J Dennis, Stuart D Dowall, Brian Getty, Donald S Anson, Michael A Skinner, James P Stewart, Tom J Blanchard

Primary Institution: Liverpool School of Tropical Medicine

Hypothesis

Can a heterologous prime-boost-boost vaccination regimen using DNA and recombinant poxvirus vectors effectively elicit an immune response against HIV-1 in Chinese cynomolgus macaques?

Conclusion

The study found that the vaccination regimen was poorly immunogenic, with only one macaque producing an HIV-specific antibody response that did not neutralize HIV-1.

Supporting Evidence

  • The vaccine candidates showed appropriate expression of recombinant proteins.
  • Only one macaque elicited an HIV-specific antibody response.
  • The antibodies did not neutralize primary isolates of HIV-1.

Takeaway

Researchers tried to create a vaccine for HIV using different methods, but it didn't work well in monkeys.

Methodology

Three macaques were immunized with a prime-boost-boost regimen using DNA vaccines and recombinant poxvirus vectors, followed by assessments of antibody and T cell responses.

Limitations

The study had a small sample size and the immunogenicity was low, with only one macaque showing an antibody response.

Participant Demographics

Three male Chinese cynomolgus macaques aged 4-5 years.

Digital Object Identifier (DOI)

10.1186/1743-422X-8-429

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