CREB Knockdown in Myeloid Leukemia Cells
Author Information
Author(s): Pellegrini Matteo, Cheng Jerry C, Voutila Jon, Judelson Dejah, Taylor Julie, Nelson Stanley F, Sakamoto Kathleen M
Primary Institution: University of California, Los Angeles
Hypothesis
If a gene is found to be differentially expressed in the CREB shRNA K562 transduced cells, and bound by CREB, it is likely to be a direct target.
Conclusion
CREB regulates a subset of histone genes that are aberrantly activated in leukemia cells, potentially contributing to the malignant phenotype.
Supporting Evidence
- CREB is overexpressed in acute myeloid leukemia cells compared to normal hematopoietic stem cells.
- CREB knockdown inhibits leukemic cell proliferation in vitro and in vivo.
- Histone genes regulated by CREB were more likely to be specifically expressed in hematopoietic lineages.
Takeaway
Scientists studied a protein called CREB in leukemia cells and found that it controls certain genes that might help the cancer grow. By turning down CREB, they saw changes in these genes.
Methodology
The study involved transducing K562 myeloid leukemia cells with CREB shRNA and performing expression profiling to identify differentially regulated genes.
Limitations
The exact number of true CREB targets is difficult to determine, and the study does not claim to identify all functional CREB targets.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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