How Salt, Heat, and Acid Affect a Probiotic Gene
Author Information
Author(s): Vittorio Capozzi, Mattia Pia Arena, Elisabetta Crisetti, Giuseppe Spano, Daniela Fiocco
Primary Institution: Department of Food Science, University of Foggia
Hypothesis
The hsp16 gene of Lactobacillus acidophilus is regulated differently by salt, high temperature, and acidic stresses.
Conclusion
The hsp16 gene of Lactobacillus acidophilus is induced by salt, high temperature, and acidic stress, but repressed by bile stress.
Supporting Evidence
- The hsp16 gene was induced by high temperatures of 45 °C and 53 °C.
- Salt stress at concentrations of 1 M and 2.5 M NaCl induced the hsp16 gene expression.
- Acidic stress at pH 4 increased hsp16 gene expression, especially with lactic acid.
Takeaway
This study looks at how a specific gene in a probiotic bacteria reacts to different stressful conditions like heat and salt. It found that the gene helps the bacteria survive these stresses.
Methodology
The study used reverse transcription quantitative PCR (RT-qPCR) to quantify the expression of the hsp16 gene under various stress conditions.
Digital Object Identifier (DOI)
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