FIP1L1-PDGFRA molecular analysis in the differential diagnosis of eosinophilia
2009

FIP1L1-PDGFRA Analysis in Eosinophilia Diagnosis

Sample size: 15 publication Evidence: moderate

Author Information

Author(s): Loules Gedeon, Kalala Fani, Giannakoulas Nikolaos, Papadakis Emmanouil, Matsouka Panagiota, Speletas Matthaios

Primary Institution: University of Thessaly Medical School, University Hospital of Larissa, Greece

Hypothesis

What is the prevalence and the associated clinicopathologic and genetic features of FIP1L1-PDGFRA rearrangement in patients with eosinophilia?

Conclusion

Early diagnosis of FIP1L1-PDGFRA-positive chronic eosinophilic leukemia and treatment with imatinib can lead to excellent clinical outcomes.

Supporting Evidence

  • Two patients with FIP1L1-PDGFRA rearrangement responded well to imatinib treatment.
  • The study found a 13.3% prevalence of FIP1L1-PDGFRA rearrangement in the cohort.
  • Patients with idiopathic hypereosinophilic syndrome also showed positive responses to imatinib.

Takeaway

This study looked at patients with high eosinophil counts to see if a specific gene change was present, which can help doctors give better treatment.

Methodology

The study used RT-PCR to detect FIP1L1-PDGFRA rearrangement and confirmed results with direct sequencing.

Limitations

The sample size was small, which may affect the generalizability of the findings.

Participant Demographics

7 males and 8 females, mean age 45.2 years (range: 22–72 years).

Digital Object Identifier (DOI)

10.1186/1471-2326-9-1

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