Fgfr2 Regulates Left-Right Asymmetry in Zebrafish
Author Information
Author(s): Liu Da-Wei, Hsu Chia-Hao, Tsai Su-Mei, Hsiao Chung-Der, Wang Wen-Pin
Primary Institution: Tzu-Chi University, Hualien, Taiwan
Hypothesis
What role does Fgfr2 signaling play in zebrafish left-right asymmetry?
Conclusion
Fgfr2c is crucial for maintaining left-right asymmetry during zebrafish development.
Supporting Evidence
- In 94.1% of wild type embryos, the liver bud was located on the left side and the pancreatic bud on the right.
- In fgfr2c morphants, 29.6% of embryos exhibited abnormal left-right asymmetry.
- The expression of lefty1 was absent in most fgfr2c morphants, indicating disruption of the molecular midline barrier.
- Cilia length was reduced in fgfr2c morphants compared to wild type embryos.
Takeaway
This study found that a specific gene, Fgfr2c, helps zebrafish develop their organs in a left-right pattern, and when it's disrupted, the organs can end up on the wrong side.
Methodology
The researchers used morpholinos to block specific fgfr2 variants and analyzed the resulting effects on organ positioning and gene expression in zebrafish embryos.
Limitations
The study primarily focused on zebrafish, which may limit the generalizability of the findings to other species.
Participant Demographics
Zebrafish embryos were used in the study.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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