Engineering Bispecificity into a Single Albumin-Binding Domain
2011
Engineering Small Bispecific Proteins for Targeting TNF-α and Albumin
publication
10 minutes
Evidence: high
Author Information
Author(s): Nilvebrant Johan, Alm Tove, Hober Sophia, Löfblom John
Primary Institution: Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
Hypothesis
Can we create a small bispecific protein that binds both TNF-α and albumin effectively?
Conclusion
The study successfully engineered small bispecific proteins that bind to both TNF-α and albumin with high affinity.
Supporting Evidence
- Two bispecific candidates were isolated with high affinity to both TNF-α and albumin.
- The smallest engineered bispecific proteins reported so far were created in this study.
- Affinity maturation led to significant improvements in binding affinities for both targets.
Takeaway
Researchers made tiny proteins that can grab onto two important targets in the body, which could help in treating diseases better.
Methodology
The study used phage display and staphylococcal surface display to engineer and select bispecific proteins.
Limitations
The study did not confirm simultaneous binding of the bispecific proteins to both targets.
Digital Object Identifier (DOI)
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