Gene Expression Changes in the Lateral Geniculate Nucleus Due to Monocular Visual Deprivation in Monkeys
Author Information
Author(s): Cheng Georgiana, Kaminski Henry J., Gong Bendi, Zhou Lan, Hatala Denise, Howell Scott J., Zhou Xiaohua, Mustari Michael J.
Primary Institution: Cleveland Clinic
Hypothesis
The anatomic and physiologic changes associated with amblyopia are regulated by genes and proteins, and characterization of changes in gene expression profiling would allow identification of important genes involved in the molecular pathogenesis of amblyopia.
Conclusion
Significant differences in gene expression profiles were found between deprived and nondeprived layers of the lateral geniculate nucleus, which may play a critical role in the molecular pathogenesis of amblyopia.
Supporting Evidence
- 116 transcripts were differentially expressed between deprived and nondeprived parvocellular layers.
- 45 genes were downregulated and 71 genes were upregulated in deprived parvocellular layers.
- 74 transcripts exhibited altered expression in deprived magnocellular laminae.
Takeaway
When monkeys were deprived of vision in one eye, their brain showed changes in the way certain genes were expressed, which might help us understand why some kids have trouble seeing.
Methodology
Monocular vision deprivation was induced in rhesus monkeys, followed by laser capture microdissection of the lateral geniculate nucleus to analyze gene expression using DNA microarray techniques.
Limitations
The study focused on a limited number of time points and may not capture all relevant gene expression changes related to amblyopia.
Participant Demographics
Infant rhesus monkeys, born in captivity and hand-raised.
Statistical Information
Statistical Significance
p<0.05
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