Regulation of Cadherin-11 Expression in Cancer Cells
Author Information
Author(s): Farina Anne K., Bong Yong-Sik, Feltes Carolyn M., Byers Stephen W.
Primary Institution: Lombardi Comprehensive Cancer Center and Departments of Oncology and Biochemistry, Molecular and Cellular Biology, Georgetown University School of Medicine
Hypothesis
How does GSK-3β regulate cadherin-11 expression in prostate and breast cancer cells?
Conclusion
Cadherin-11 mRNA and protein levels are regulated by the activity of GSK3β, with significant regulation exerted by β-catenin at the level of the cadherin-11 3′UTR.
Supporting Evidence
- GSK3β regulates cadherin-11 expression in two ways: through β-catenin-independent regulation of mRNA levels and β-catenin-dependent effects on 3′UTR stability.
- Inhibition of GSK3β with lithium chloride or BIO reduced cadherin-11 mRNA and protein levels.
- Cadherin-11 3′UTR is highly conserved and plays a significant role in its regulation.
Takeaway
This study shows that a protein called GSK3β helps control the levels of another protein, cadherin-11, which is important in cancer cells.
Methodology
The study used various cancer cell lines and treatments to analyze the effects of GSK3β inhibition on cadherin-11 expression.
Limitations
The study primarily focuses on specific cancer cell lines and may not generalize to all cancer types.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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