Muscarinic Receptor Expression in Tree Shrew Eyes and Myopia Development
Author Information
Author(s): McBrien N.A., Jobling A.I., Truong H.T., Cottriall C.L., Gentle A.
Primary Institution: Department of Optometry and Vision Sciences, the University of Melbourne, Victoria, Australia
Hypothesis
The study investigates the expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia.
Conclusion
Muscarinic receptor gene expression does not change during the early stages of myopia development in tree shrew ocular tissues.
Supporting Evidence
- All five muscarinic receptor subtypes were expressed in the iris/ciliary body, retina, choroid, and sclera.
- Gene expression data were confirmed by immunohistochemistry.
- Muscarinic receptor gene expression remained unaltered in the retinal, choroidal, and scleral tissue samples after one or five days of myopia development.
Takeaway
The study looked at how certain receptors in the eyes of tree shrews behave when myopia starts, and found that these receptors don't change much even when myopia develops.
Methodology
Total RNA was isolated from various ocular tissues, and muscarinic receptor expression was assessed using RT-PCR and immunohistochemistry.
Limitations
The study only examined early stages of myopia and did not assess long-term changes in receptor expression.
Participant Demographics
Maternally reared tree shrews (Tupaia belangeri) aged 15 days post eye opening.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
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