Transient Receptor Potential (TRP) and Cch1-Yam8 Channels Play Key Roles in the Regulation of Cytoplasmic Ca2+ in Fission Yeast
2011
Key Roles of TRP and Cch1-Yam8 Channels in Calcium Regulation in Fission Yeast
publication
10 minutes
Evidence: high
Author Information
Author(s): Ma Yan, Sugiura Reiko, Koike Atsushi, Ebina Hidemine, Sio Susie O., Kuno Takayoshi
Primary Institution: Kobe University Graduate School of Medicine
Hypothesis
How do TRP and Cch1-Yam8 channels regulate cytoplasmic Ca2+ levels in fission yeast?
Conclusion
The study found that TRP and Cch1-Yam8 channels play crucial roles in regulating cytoplasmic Ca2+ levels in fission yeast.
Supporting Evidence
- The study demonstrated that extracellular Ca2+ addition caused a dose-dependent increase in cytoplasmic Ca2+ levels.
- Overexpression of TRP channels Trp1322 and Pkd2 significantly enhanced the Ca2+ response.
- Gene deletions affecting calcineurin and Prz1 altered the Ca2+ signaling response.
- NaCl plus FK506 treatment resulted in a persistent increase in cytoplasmic Ca2+ levels.
Takeaway
This study shows that certain channels in yeast help control calcium levels, which are important for the cell's health and function.
Methodology
The study used a bioluminescence resonance energy transfer-based assay with a GFP-aequorin fusion protein to monitor cytoplasmic Ca2+ levels in living fission yeast cells.
Digital Object Identifier (DOI)
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