Endocrine Disruption in Coastal Fish
Author Information
Author(s): Michael E. Baker, Barbara Ruggeri, L. James Sprague, Colleen Eckhardt-Ludka, Jennifer Lapira, Ivan Wick, Laura Soverchia, Massimo Ubaldi, Alberta Maria Polzonetti-Magni, Doris Vidal-Dorsch, Steven Bay, Joseph R. Gully, Jesus A. Reyes, Kevin M. Kelley, Daniel Schlenk, Ellen C. Breen, Roman Šášik, Gary Hardiman
Primary Institution: University of California, San Diego
Hypothesis
Can a multispecies microarray effectively screen for endocrine disruptors in coastal fish?
Conclusion
The multispecies microarray is a valid tool for detecting endocrine disruptors in fish from polluted environments.
Supporting Evidence
- The microarray detected altered gene expression profiles in turbot from polluted areas.
- Zebrafish exposed to estradiol and nonylphenol showed significant changes in gene expression.
- The study highlights the utility of the microarray for monitoring environmental chemicals.
- Control fish had significantly lower vitellogenin levels compared to those from polluted sites.
Takeaway
Scientists created a special tool to check if fish are getting sick from chemicals in the water, and it worked well.
Methodology
The study used a custom multispecies microarray and qRT-PCR to analyze gene expression in fish collected from polluted and clean waters.
Limitations
Limited genomic information for turbot and other flatfish restricted the use of molecular tools.
Participant Demographics
Male hornyhead turbot and zebrafish were used in the study.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website