RNA Modulators of Complex Phenotypes in Mammalian Cells
Author Information
Author(s): Lai Angela, Cairns Murray J., Tran Nham, Zhang Hong-Ping, Cullen Lara, Arndt Greg M.
Primary Institution: Johnson and Johnson Research Pty Ltd, Australian Technology Park, Eveleigh, New South Wales, Australia
Hypothesis
Can a random small RNA expression library identify small interfering RNA effectors that modify complex cellular phenotypes in mammalian cells?
Conclusion
The study successfully identified small RNA effectors that can overcome cell death induced by chemotherapeutic agents in colorectal cancer cells.
Supporting Evidence
- The random small RNA library identified 430 and 500 resistant clones after treatment with 5-FU and TNF-α, respectively.
- One specific insert (5FU55) was enriched in 55 independent 5-FU resistant clones.
- Cells transfected with synthetic 5FU55 siRNA showed increased resistance to 5-FU in a dose-dependent manner.
Takeaway
Researchers created a special library of tiny RNA pieces to find ones that help cells survive when they are treated with cancer drugs.
Methodology
The study involved constructing a random small RNA expression library and using it in genetic selection assays to identify RNA effectors.
Limitations
The complexity of the library was low, and the identified RNA effectors did not have complete complementarity with known target transcripts.
Participant Demographics
The study focused on HCT116 colorectal cancer cells.
Digital Object Identifier (DOI)
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