Creation of a New Mouse Model for Studying Sensory Neurons
Author Information
Author(s): Zurborg Sandra, Piszczek Agnieszka, MartÃnez Conception, Hublitz Philip, Al Banchaabouchi Mumna, Moreira Pedro, Perlas Emerald, Heppenstall Paul A
Primary Institution: Mouse Biology Unit, European Molecular Biology Laboratory (EMBL)
Hypothesis
Can we create a Cre-driver mouse line that specifically targets peripheral sensory neurons?
Conclusion
The Advillin-Cre-driver mouse line effectively targets peripheral sensory neurons, making it a valuable tool for future research.
Supporting Evidence
- The Advillin-Cre line showed almost exclusive Cre-mediated recombination in peripheral sensory neurons.
- Behavioral tests indicated normal nociceptive responses in Advillin-Cre mice compared to wildtype.
- Copy number analysis revealed five copies of the transgene per genome in the Advillin-Cre mice.
Takeaway
Scientists made a special mouse that helps them study pain and touch nerves better. This mouse can help find out how these nerves work.
Methodology
The study involved generating a BAC-transgenic mouse expressing Cre-recombinase from the Advillin gene and characterizing its expression and functionality.
Potential Biases
Potential bias in interpreting behavioral results due to the specific targeting of sensory neurons.
Limitations
The study did not explore the effects of Cre expression in all potential neuronal subpopulations.
Participant Demographics
The study involved adult mice and embryos at various developmental stages.
Statistical Information
P-Value
0.151
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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