Chemometric analysis of biofluids from mice experimentally infected with Schistosoma mansoni
2011

Metabolic Changes in Mice Infected with Schistosoma mansoni

Sample size: 20 publication 10 minutes Evidence: moderate

Author Information

Author(s): Li Jia V, Saric Jasmina, Wang Yulan, Keiser Jennifer, Utzinger Jürg, Holmes Elaine

Primary Institution: Imperial College London

Hypothesis

What are the metabolic changes in mice infected with Schistosoma mansoni over time?

Conclusion

The study identified specific metabolic changes in urine, plasma, and faecal samples of mice infected with Schistosoma mansoni, providing insights into host-parasite interactions.

Supporting Evidence

  • Urinary biomarkers such as hippurate and phenylacetylglycine were identified as indicators of infection.
  • Changes in plasma metabolites reflected alterations in energy metabolism due to the infection.
  • Faecal metabolite profiles showed variability, complicating the identification of consistent biomarkers.

Takeaway

When mice get infected with a parasite called Schistosoma mansoni, their body fluids change in specific ways that can help scientists understand how the infection affects them.

Methodology

The study involved infecting 20 female NMRI mice with cercariae and analyzing their urine, plasma, and faecal samples using NMR spectroscopy over a 73-day period.

Potential Biases

Potential bias due to the small sample size and the specific strain of mice used.

Limitations

The variability in faecal metabolite composition made it difficult to draw clear conclusions from faecal samples.

Participant Demographics

20 female NMRI mice, aged 5 weeks.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1756-3305-4-179

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